SAT Biology Practice Test 8: DNA Technology

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Question 5 questions

Time 4 minutes

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1. Which of these can be used to increase the amount of DNA available for analysis?

I. Cloning a plasmid

II. Gel electrophoresis

II. PCR

A. II only
B. I and II
C. I and III
D. II and III
E. I, II, and III

2. A plasmid containing a normal human beta-globin allele is digested with restriction enzymes. The results are shown in lane 2 of the electrophoresis gel. A second plasmid, contain the beta-globin allele that results in sickle-cell disease, is digested in the same way and placed in lane 3. The standard in lane 1 contains DNA fragments in lengths of 100 base pairs (bp), 200 bp, and so on.

Which of these describes the results shown on the gel?

A. Both lanes 1 and 2 both contain 100-bp fragments.
B. Only lane 2 contains a 175-bp fragment.
C. Only lane 3 contains a 375-bp fragment.
D. Both lanes 2 and 3 contain two equal-sized fragments.
E. Only lane 3 contains a 300-bp fragment.

3. A plasmid containing a normal human beta-globin allele is digested with restriction enzymes. The results are shown in lane 2 of the electrophoresis gel. A second plasmid, contain the beta-globin allele that results in sickle-cell disease, is digested in the same way and placed in lane 3. The standard in lane 1 contains DNA fragments in lengths of 100 base pairs (bp), 200 bp, and so on.

Based on the results shown, which of these BEST describes the mutation that resulted in the sickle-cell allele?

A. It created a site that is recognized by the restriction enzyme.
B. It removed two restriction sites recognized by the enzyme.
C. It changed a restriction site cut by the restriction enzyme.
D. It inserted a second restriction site specific for the enzyme.
E. It inserted a third restriction site specific for the enzyme.

4. A plasmid is added to bacteria cells, which are then spread on plates containing nutrient media. In order to select for cells that took up the plasmid, the

A. plasmid should contain an auxotrophic mutation, and the medium should contain the missing nutrient
B. plasmid should contain a gene for producing a nutrient, and the bacterial chromosome should contain an auxotrophic mutation
C. bacterial chromosome should contain an antibiotic resistance gene, and the medium should contain the antibiotic
D. plasmid should contain a gene for producing an antibiotic, and the bacterial chromosome should contain a gene for antibiotic resistance
E. bacterial chromosome should contain an auxotrophic mutation, and the medium should contain the missing nutrient

5. Which DNA technique depends, in part, on a violation of Mendel's law of independent assortment?

A. Shotgun genome sequencing
B. RFLP
C. PCR
D. Plasmid cloning and selection
E. Genome marker mapping